Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  : 10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity. 
It was decades later that the secret behind this spectacular success became known. The East German Sports Federation had, with the help of the Stasi, used Performance Enhancing Drugs or PEDs to ensure that their athletes gained international recognition by winning the Olympic events. This systematic plan had been initiated in 1974 as a means to guarantee international glory through the achievement of gold medals at the prestigious sporting event. Oral- Turinabol , a testosterone derivative was used extensively to improve muscle mass and cut down recovery time. This allowed the German athletes to train harder and longer than other world athletes.